As a result of recent combat operations in Iraq and Afghanistan, the military has been entrusted to care for over 1,700 service members and veterans who have experienced traumatic limb loss, 82.7% of which have been of the lower limb. Beyond the physical and psychological difficulties of adapting to life after limb loss, individuals with amputations are at a higher risk for developing secondary complications, such as joint pain, osteoarthritis, chronic back pain, cardiovascular disease, and phantom limb pain., Optimizing rehabilitation strategies in caring for service members with amputation may not only improve their functional performance, but will also likely mitigate many of these long-term risks. While the Department of Defense has established three Advanced Rehabilitation Centers (ARCs) that specialize in a state-of-the-art interdisciplinary rehabilitative care utilizing the latest technology in prosthetics and assistive devices, further research is needed to help optimize those rehabilitation interventions, particularly as related to establishing individualized guidelines for prescribed intensity, duration, and frequency of exercise regiments. Near infrared spectroscopy (NIRS) is currently used to measure localized muscle oxidative metabolism and hemodynamics during exercise; however, this technology has not yet been assessed for the potential to generate patient-specific rehabilitation regimens. This study was intended to examine the feasibility of wearing the NIRS system during rehabilitation of service members and veterans with lower limb loss (transtibial and transfemoral).